Carnoy's fixative ntxiv chloroform thiab acetic acid rau qhov sib tovuas tawm tsam cov teebmeem ntawm ethanol thiab ua rau cov ntaub so ntswg fixation los ntawm hydrogen bonding ntawm cov khoom rau cov ntaub so ntswg [2].
Vim li cas Carnoy cov kua dej siv hauv mitosis?
Thaum cov ntaub so ntswg khaws cia hauv acetic acid rau lub sijhawm ntev, acetic acid dissolves histones ntawm chromosomes ua rau lawv degradation. Carnoy's kuv yog feem ntau siv fixative thiab muab cov txiaj ntsig zoo rau ntau hom sib txawv thiab cov ntaub so ntswg.
fixative siv rau dab tsi?
Kev ua haujlwm ntawm Fixative
Lub luag haujlwm tseem ceeb ntawm kev kho yogtiv thaiv autolysis (enzymes nres) nrog rau putrefaction (bacterial attack) ntawm cov ntaub so ntswg.
Qhov zoo ntawm kev siv acetic acid li fixative yog dab tsi?
Acetic acid
Nws yog suav nrog hauv cov tshuaj kho kom zoo rau pab tiv thaiv kom tsis txhob poob ntawm nucleic acidsthiab, vim nws swells collagen, los tiv thaiv qhov shrinkage los ntawm lwm yam. cov khoom xyaw xws li ethanol. Acetic acid nkag mus sai heev tab sis cov tshuaj kho uas muaj nws yuav ua rau cov ntshav liab lyse.
Vim li cas Bouin cov kua dej siv?
Kev siv lub ntsiab ntawm Bouin cov kua yog kho cov qog nqaij hlav, prostate thiab lub raum biopsies. Nws yog ib qho zoo heev fixative thaum mos thiab muag muag cov ntaub so ntswg yuav tsum tau khaws cia ntawm qhov tod tes nws tsis pom zoo kom siv nws los kho cov ntaub so ntswg.rau electron microscopy.
