Txoj Kev Schaeffer-Fulton yog cov txheej txheem feem ntau siv endospore staining, uas siv Malachite ntsuab ua thawj stain. Thaum lub endospore tau nqus cov stains, nws yog resistant rau decolorization, tab sis lub vegetative cell yog yooj yim decolorized nrog dej (tawm cov vegetative cells tsis muaj xim).
malachite ntsuab stain siv rau dab tsi?
Malachite Green yog siv rau kab mob kis kab mob los ntawm Schaeffer thiab Fulton txoj kev. Nws kuj tseem siv tau los ua ib qho yooj yim stain rau cov kab mob hlwb thiab nyob rau hauv qhov chaw ntawm methyl ntsuab. Spore yog ib hom kab mob dormant uas tso cai rau nws kom muaj sia nyob hauv ib puag ncig hnyav.
Qhov twg stain siv malachite ntsuab reagent?
Malachite Green 1% w/v yog siv los ua cov tshuaj staining hauv spores staining thiab staining yooj yim. Malachite Green yog siv rau cov kab mob spores staining los ntawm Schaeffer thiab Fulton's txoj kev. Nws kuj tseem siv tau los ua ib qho yooj yim stain rau cov kab mob hlwb thiab qhov chaw ntawm methyl-ntsuab hauv Pappenheim stain, thaum ua ke nrog Gram stain.
Puas malachite ntsuab yog qhov thib ob?
Ntawm no, nws tsim nyog sau cia tias thawj qhov stain thiab cov stain thib ob yog cov xim sib txawv. Yog li ntawd, lawv tso cai rau tus kws kho mob kom sib txawv ntau hom hlwb nyob rau hauv lub tshuab tsom. Whereas lub counterstain (safranin) yog xim liab / reddish nyob rau hauv cov xim, cov thawj stain (malachite ntsuab) yog ntsuab xim.
Ibmalachite ntsuab ib tug thawj stain?
Nyob rau hauv Schaeffer-Fulton txoj kev, thawj stain-malachite ntsuab raug yuam mus rau hauv lub spores los ntawm steaming cov kab mob emulsion. Malachite ntsuab yog dej soluble thiab muaj kev sib raug zoo rau cov khoom siv cellular, yog li cov noob qoob loo tuaj yeem decolourized nrog dej. … Spore zoo kuj tseem siv rau kev kuaj mob.
