Cov txheej txheem ntawm kev tsim cov somatic hybrids (fusion ntawm hlwb ntawm ob hom ntawm hom) los ntawm polyethylene glycol (PEG) yog hu ua somatic hybridization. Cov nroj tsuag tsis muaj cell phab ntsa hu ua protoplast. Kev sib cais ntawm protoplasts yog ua los ntawm kev kho cov hlwb nrog pectinase thiab cellulase enzymes.
Cov tshuaj twg siv rau hauv somatic hybridization?
Yuav tsim cov hybrids somatic ntawm ntau thiab Tartary buckwheat, mesophyll protoplasts ntawm F. esculentum tau fused los ntawm polyethylene glycol-mediated fusion nrog hypocotyl protoplasts ntawm Fagopyrum tataricum, ua tus hauler (Lachmanni li al., 1994).
Dab tsi yog somatic cell hybrids?
Somatic cell hybrids yog kab lis kev cai uas muaj tag nrho cov ntxiv ntawm nas genome thiab ob peb tib neeg chromosomes. Cov kab lis kev cai no yog tsim los ntawm kev sib xyaw tib neeg thiab nas hlwb nyob rau hauv muaj tus kab mob Sendai. Tus kab mob no pab txhawb kev sib txuas ntawm ob hom cell los tsim ib lub xov tooj sib txuas.
Yuav ua li cas yog cov kauj ruam yooj yim koom nrog hauv somatic hybridization?
Cov kauj ruam tseem ceeb hauv cov txheej txheem ntawm kev sib xyaw somatic yog: (1) kev sib cais ntawm protoplasts, (2) fusion ntawm protoplasts, (3) kab lis kev cai ntawm protoplasts los tsa tag nrho cov nroj tsuag, (4) kev xaiv ntawm hybrid hlwb thiab kev txheeb xyuas hybridity Page 5 ISOLATION OF PROTOPLAST Protoplast tuaj yeem raug cais tawm ntawm yuav luag txhua qhov chaw cogi.e.…
Peb yam ntawm somatic hybridization yog dab tsi?
Lub somatic hybridization muaj peb yam. Peb yam yog: (A) Fusion ntawm Protoplasts (B) Kev Xaiv Cov Cells Hybrid thiab (C) Kev Qhia Txog Cov Nroj Tsuag hybrid. Txoj hauv kev los txhim kho cov yam ntxwv ntawm cov nroj tsuag cog, tau ntau xyoo, tau sib deev hybridization.