Gelatin hydrolysis test yog siv los kuaj xyuas lub peev xwm ntawm lub cev los tsim cov gelatinases uas liquefy gelatin. Cov txheej txheem no tshwm sim nyob rau hauv ob qhov kev tshwm sim ua ntu zus. Hauv thawj cov tshuaj tiv thaiv, gelatinases degrade gelatin rau polypeptides. Tom qab ntawd, cov polypeptides tau hloov dua siab tshiab rau hauv cov amino acids.
Lub hom phiaj ntawm kev kuaj gelatinase yog dab tsi?
Kev kuaj gelatinase tuaj yeem yog siv los sib txawv ntawm Staphylococcus aureus thiab Staphylococcus epidermidis. Nws kuj tuaj yeem siv los sib txawv Serratia marcescens, Proteus vulgaris, thiab Proteus mirabilis los ntawm lwm yam enterics.
Yuav ua li cas thiaj ua tiav qhov kev xeem gelatinase?
Principle of Gelatin Hydrolysis Test
Kev kuaj pom muaj gelatinases tau kuaj pom siv cov khoom noj khoom haus gelatin nruab nrab. Qhov nruab nrab no yog ib qho yooj yim nruab nrab ntawm gelatin, peptone thiab nqaij nyuj extract. … Thaum cov kab mob tsis zoo gelatinase tsis zais cov enzymes thiab tsis liquefy nruab nrab.
Dab tsi enzyme Liquifies gelatin?
Gelatin yog cov protein uas muaj zog ntawm chav tsev kub. Yog tias cov kab mob ua rau enzyme gelatinase(uas zoo tshaj yog tsim ntawm 25 ° C, tsis yog 37 ° C), gelatin yog hydrolyzed thiab dhau los ua kua.
Dab tsi yog qhov substrate ntawm gelatin hydrolysis test?
Nyob hauv nutrient gelatin, gelatin yog qhov nruab nrab tus neeg sawv cev thiab cov substrate rau cov tshuaj biochemical no. Yog hais tias inoculatedkab mob tsim gelatinases, cov protein yuav tawg mus rau hauv me me polypeptides thiab amino acids, liquefying nruab nrab.
