Cov khoom tseem ceeb ntawm MacConkey nruab nrab suav nrog crystal violet dye, bile ntsev, lactose, thiab nruab nrab liab (pH qhia). Crystal violet dye thiab bile ntsev txwv kev loj hlob ntawm cov kab mob gram-zoo. Qhov no tso cai rau tsuas yog hom kab mob tsis zoo los tsim cov cheeb tsam ntawm MAC agar. … Cov lactose hauv agar yog qhov chaw fermentation.
Puas E coli loj hlob ntawm MacConkey agar?
Kev xaiv thiab sib txawv
MacConkey agar tsis tsuas yog xaiv rau cov kab mob Gram-negative los ntawm inhibiting Gram-positive kab mob thiab cov poov xab tab sis kuj txawv cov kab mob Gram-tsis zoo los ntawm lactose fermentation. … Escherichia coli thiab lwm yam lactose ferments yuav tsim daj lossis txiv kab ntxwv kab.
Vim li cas MacConkey agar siv rau E coli?
Sorbitol MacConkey agar yog ib qho txawv ntawm ib txwm siv MacConkey agar siv hauv kev tshawb nrhiav E. coli O157:H7. … Qhov no tseem ceeb heev vim cov kab mob plab, xws li Escherichia coli, feem ntau tuaj yeem ferment lactose, thaum cov kab mob tseem ceeb hauv plab, xws li Salmonella enterica thiab shigellas feem ntau tsis tuaj yeem ferment lactose.
Cov ntaub ntawv ntawm MacConkey media yog dab tsi?
MacConkey agar muaj plaub cov khoom xyaw tseem ceeb (lactose, bile ntsev, crystal violet, thiab nruab nrab liab)uas ua rau nws xaiv thiab sib txawv xov xwm. Bile ntsev thiab siv lead ua violet ua raws li cov neeg ua haujlwm xaiv uas inhibit qhov kev loj hlob ntawm Gram-zoo kab mob, thiab proliferate cov kev xaiv loj hlob ntawm gram-tsis zoo.kab mob.
Yuav ua li cas ua agar nutrient?
Yuav ua li cas npaj zaub mov agar?
- Suspend 28g ntawm nutrient agar hmoov (CM0003B) hauv 1L ntawm cov dej distilled.
- Sib tov thiab yaj lawv tag.
- Sterilize los ntawm autoclaving ntawm 121 ° C rau 15 feeb.
- Muab cov kua tso rau hauv lub tais petri thiab tos kom nruab nrab kom khov.
