Tetrazine ligation yog cov tshuaj tiv thaiv ntawm trans-cyclooctene thiab s-tetrazine nyob rau hauv qhov kev thov rov qab Diels Alder cov tshuaj tiv thaiv ua raws li cov tshuaj tiv thaiv retro-Diels Alder kom tshem tawm cov roj nitrogen. … Yog li ntawd, cov strained heev trans-cyclooctene yog siv raws li ib tug reactive dienophile.
Dab tsi yog bio orthogonality?
Bioorthogonal yog txhais tias tsis cuam tshuam lossis cuam tshuam nrog biology. Cov tshuaj tiv thaiv uas yog bioorthogonal tsis ntev los no tau dhau los ua cov cuab yeej tseem ceeb rau kev pom cov biomolecules hauv lawv qhov chaw ib puag ncig, tshwj xeeb yog cov uas tsis tuaj yeem hloov kho cov caj ces ib txwm muaj.
Cov tshuaj tiv thaiv orthogonal yog dab tsi?
Lub tswv yim ntawm orthogonality tau siv rau ntau qhov chaw ntawm chemistry, xws li kev ua haujlwm nthwv dej mus rau chromatography. … Cov tshuaj tiv thaiv nyem thiab nws cov variants raug suav hais tias yog orthogonal li cov khoom sib xyaw ua ke hauv cov txiaj ntsig siab thiab muaj ntau lwm pab pawg ua haujlwm.
Lub hom phiaj ntawm bioorthogonal chemistry yog dab tsi?
Qhov dav ntawm bioorthogonal cov tshuaj tiv thaiv nrog lawv cov reactants cuam tshuam, cov reagents tseem ceeb, cov khoom thiab cov yam ntxwv tseem ceeb tau hais txog ntawm no. Bioorthogonal chemistry ua kom muaj kev soj ntsuam cov kab mob lom neeg los ntawm kev xaiv covalent daim ntawv cog lus uas cuam tshuam tsawg kawg rau cov txheej txheem uas tau kawm.
TCO chemistry yog dab tsi?
Description: Lub inverse-electron thov Diels-Alder cycloaddition cov tshuaj tiv thaiv ntawm trans-Cyclooctenes (TCO) nrog tetrazines yog bioorthogonal cov tshuaj tiv thaiv uas muaj qhov tshwj xeeb kinetics (k > 800 M-1s- 1) thiab kev xaiv. … Cov tshuaj tiv thaiv TCO-tetrazine nyem yog tus qauv hauv cov protein-protein conjugation.
