Lub teeb liab polyadenylation - cov kab ke motif lees paub los ntawm RNA cleavage complex- sib txawv ntawm pab pawg ntawm eukaryotes. … Qhov chaw cleavage txuam nrog lub teeb liab polyadenylation tuaj yeem sib txawv txog li 50 nucleotides. Thaum lub RNA yog cleaved, polyadenylation pib, catalysed los ntawm polyadenylate polymerase.
Lub hom phiaj ntawm lub teeb liab polyadenylation yog dab tsi?
Lub hom phiaj thiab cov txheej txheem ntawm polyadenylation sib txawv ntawm cov xovtooj ntawm tes, tab sis polyadenylation feem ntau ua haujlwm rau txhawb nqa cov ntawv sau ntev hauv eukaryotes thiab txhawb nqa cov ntawv degradation hauv prokaryotes.
Qhov teeb meem polyadenylation nyob qhov twg pom?
mRNA cleavage nyob rau hauv cov tsiaj yog xav tias yuav tswj tau los ntawm ob lub ntsiab lus tseem ceeb, cov teeb liab polyadenylation zoo (PAS) nyob 10–30 bases upstream ntawm mRNA cleavage site (CS)thiab qhov kev txuag tsawg dua U-nplua nuj, hu ua downstream sequence element (DSE) thiab nyob hauv thawj 30 nucleotides…
Qhov teeb meem polyadenylation nyob qhov twg feem ntau nyob hauv cov noob?
Nyob rau hauv cov kab mob mammalian, zoo polyadenylation yuav tsum muaj ob lub ntsiab sib lawv liag: ib tug heev conserved AAUAAA teeb liab nyob 10–30 nucleotide 5′ rau qhov chaw cleavagethiab ib tug ntau variable GU- cov khoom nplua nuj, 20–40 hauv paus 3′ ntawm qhov chaw (saib Proudfoot 1991; Colgan thiab Manley 1997 rau kev tshuaj xyuas).
Dab tsi yog qhov ntau tshajKev pom zoo ua ntu zus rau polyadenylation?
Yuav luag tag nrho cov eukaryotic polyadenylation cov cim muaj cov ntsiab lus tseem ceeb,kev pom zoo ua ntu zus AAUAAA (los yog qhov sib txawv) ~ 10-35 nucleotides ntws ntawm qhov chaw tiag tiag ntawm poly(A) ntxiv(saib nqe 6–7, 12).
