2024 Tus sau: Elizabeth Oswald | [email protected]. Kawg hloov kho: 2024-01-13 00:07
Cov khoom ntawm kev txwv kev zom zaub mov tuaj yeem khaws tau yooj yim at -20 C. Thaum 4 C nws yuav zoo tab sis kom ntseeg tau tias tsis muaj kev ua si thiab tsis muaj hnub qub ua si nws yog pom zoo kom khaws cia ntawm -20 C.
Koj khaws DNA zom tau li cas?
sim khaws cov gel hlais hauv lub tub yees thaum hmo ntuj, lossis tseem yaj cov hlais hauv qhov tsis muaj thiab khov nws ntawm -20 ° C lossis -80 ° C. Kev degradation ntawm DNA yuav tshwm sim ntawm tus nqi tib yam thiab nyob rau hauv tib yam li cov DNA soluble, yog li siv lub txias txias kom ntev cia.
txhais li cas rau plasmid kom zom tau?
Purified plasmid DNA yog zom nrog 1 lossis ntau dua txwv enzymes (REs) xaiv los muab cov qauv DNA sib txawv uas daws tau yooj yim los ntawm electrophoresis. … Kev txwv cov enzymes uas txiav nyob rau hauv ntau qhov chaw cloning (MCS) thiab ua rau ib qho kev kuaj mob ntawm 2-5 yooj yim los daws cov bands feem ntau xaiv.
Koj khaws cov plasmids linearized li cas?
Yog tias koj txhawj xeeb txog cov teebmeem hauv qab ntawm TE, khaws cov DNA ntawm siab dua thiab dilute thaum xav tau, lossis siv "TE qis" uas yog 10 mM Tris / 0.1 hli EDTA. thaum DNA lossis RNA huv lawm nws tuaj yeem khaws cia ntawm -20C rau ntau lub hlis yam tsis muaj kev puas tsuaj.
Ntev npaum li cas koj tuaj yeem khaws cov kev txwv txiav?
Pro-Tip Nyob ntawm daim ntawv thov thiab tus nqi ntawm DNA hauv cov tshuaj tiv thaiv, lub sij hawm incubation tuaj yeem nyob ntawm 45 mins mus rau hmo ntuj. Rau kev kuaj mob, 1-2 teev yog feem ntau txaus. Rau kev zom nrog >1 µg ntawm DNA siv rau cloning, nws raug nquahu kom koj zom tsawg kawg 4 teev.
Pom zoo:
Koj puas yuav khaws cov ntawv teev npe?
Khaws daim ntawv teev npe muaj muaj peev xwm txo peb txoj kev ntxhov siabthiab kho peb cov hlab ntsha hauv cov xwm txheej ntxhov siab. Txhawm rau khaws daim ntawv teev npe ntawm koj qhov kev xav thiab kev xav nyob ib puag ncig cov xwm txheej uas koj tsis xis nyob nrog, ua rau koj tsim kev tswj hwm thiab yog li txo koj txoj kev ntxhov siab.
Akeeba khaws cov ntaub ntawv khaws cia nyob qhov twg?
Qhov chaw nyob ntawm lub nplaub tshev uas Akeeba Backup tso tawm koj cov ntaub ntawv thaub qab ib txwm administrator/components/com_akeeba/backup. Akeeba thaub qab nyob qhov twg? Cov ntaub ntawv thaub qab yog qhov uas koj hais rau Akeeba Backup kom muab tso rau lawv, piv txwv li hauv Cov Ntawv Qhia Tawm koj tau teev tseg hauv nplooj ntawv teeb tsa.
Puas khaws cia lossis khaws cia?
Lo lus khaws yog txhais tias "kom muaj lossis khaws cia," ntawm ib qho khoom, lossis "tso khoom" rau qhov chaw. Lub caij nyoog dhau los thiab yav dhau los ntawm lo lus khaws is "keep". Thaum siv tau yog? Raws li kuv hais saum toj no, yog thiab yav dhau los, tab sis lawv siv txawv.
Puas cov valet khaws koj cov yuam sij?
Feem ntau koj yuav tso cov yuam sij nrog lub valet. Qhov no yog ib feem ntawm qhov kev thov rov hais dua, koj tuaj yeem muab cov yuam sij rau lub valet thiab taug kev mus rau hauv koj qhov chaw. Cov valets sib txawv yuav muaj ntau txoj cai, tab sis feem ntau yog tias koj hais kom lawv xa cov yuam sij rau koj, lawv yuav ua.
Vim li cas cov neeg khaws nyiaj khaws cia them nyiaj?
Ib daim tshev nyiaj yog txoj kev them nyiaj nyab xeeb, muaj txiaj ntsig zoo thaum muaj nyiaj ntau, feem ntau txhua yam tshaj $ 1,000, yuav tsum tau. Qee qhov kev hloov pauv yuav xav tau tus neeg txais nyiaj daim tshev rau kev them nyiaj. Tej zaum koj yuav xav tau ib daim tshev nyiaj los ua kom muaj kev ruaj ntseg ntawm chav tsev, piv txwv li, los yog them cov nqi qis ntawm lub tsheb tshiab.
