Human Dermal Fibroblasts (HDF) yog lub luag haujlwm rau kev tsim cov cellular matrix tsim cov ntaub so ntswg ntawm daim tawv nqaij, thiab ua lub luag haujlwm tseem ceeb thaum kho qhov txhab. HDF los ntawm Cell Applications, Inc. … Lwm tus cia siab rau lub hlwb kom ua tus cwj pwm mitochondrial metabolism, angiogenesis thiab cov ntaub so ntswg kho.
Dab tsi yog tib neeg dermal fibroblasts?
Ntau Tib Neeg Dermal Fibroblasts (NHDF) yog cells cais tawm ntawm tib neeg daim tawv nqaij rau siv ntau yam kev tshawb fawb. Los ntawm daim tawv nqaij laus mus rau qhov txhab kho thiab txawm tias kev tshawb fawb qog noj ntshav, NHDF nthuav qhia ntau lub sijhawm rau kev sim hauv vitro, ob qho tib si raws li kab lis kev cai monolayer thiab hauv 3D daim tawv nqaij qauv.
tib neeg dermal fibroblasts tuaj qhov twg?
Tib neeg daim tawv nqaij fibroblasts yog mesenchymal / stromal hlwb muab tau los ntawm the embryonic mesoderm. Lawv nyob rau hauv lub dermal txheej ntawm daim tawv nqaij, qhov chaw uas lawv tsim extracellular matrix proteins kom ntxiv dag zog rau lub dermal compartment thiab cuam tshuam nrog epidermal hlwb.
Koj kab lis kev cai tib neeg fibroblasts li cas?
- Tshem tawm Fibroblasts los ntawm qhov chaw tsim khoom, tso kab lis kev cai hauv lub txee biosafety thiab aspirate media.
- Ntxiv 2 mL ntawm PBS siv 5ml serological pipette, swirl kab lis kev cai nkoj, aspirate thiab pov tseg PBS.
- Ntxiv 2 mL ntawm 0.25% Trypsin thiab incubate rau 00:05:00 ntawm 37 ° C thiab 5% CO2. Cov hlwb ua kom ruaj khov tuaj yeem raug tshem tawm sai ntawm 37 ° C.
Dab tsi yog txoj haujlwm ntawm fibroblasts hauvdaim tawv nqaij?
Fibroblasts. Fibroblasts synthesize collagen thiab extracellular matrix Cheebtsam thiab ua haujlwm hauv tsev thiab kho cov txheej txheem ntawm daim tawv nqaij. Lawv tau muab los ntawm mesoderm thiab pom thoob plaws hauv lub dermis.
